超级稻品种中嘉早17产量相关性状QTL定位研究

doi:10.16819/j.1001-7216.2016.5061

摘要/Abstract

摘要：

以中嘉早17/D50 F₂和F_2:3群体为材料,考查各群体株系产量相关农艺性状,同时构建其遗传连锁图谱,最后定位到与产量性状相关的66个QTL,其贡献率变幅为0.08% ~ 20.45%,分布于除第9染色体外的其他各染色体上。两个群体在第3、7、10染色体某区段同时定位到多个与产量性状相关的QTL, 这些QTL的加性效应来自中嘉早17。在定位到的66个QTL中,F₂和F_2:3群体均检测到,且贡献率较大的QTL qPH-10(株高)、qFLW-4(剑叶宽)、qTGW-2(千粒重) 加性效应均来自中嘉早17。初步检测到超级稻品种中嘉早17携带多个高产QTL,为今后将中嘉早17的优良基因导入其他水稻品种进而培育产量更高、综合性状更优良的超级稻新品种提供理论和技术支持。

关键词: QTL 定位, 产量性状, 超级稻

Abstract:

The rice yield related agronomic traits were examined, and the genetic linkage maps were constructed with the F₂ populations derived from Zhongjiazao 17 × D50. A total of 66 QTLs for yield related traits were mapped, with the contribution rate ranging from 0.08%~20.45%, distributed on all the 12 chromosomes except for chromosome 9. Numbers of QTLs for yield related traits were mapped in the same intervals on Chr.3, Chr.7 and Chr.10,respectively, in F₂ or F_2:3 populations, and the positive alleles were contributed by Zhongjiazao 17. Among the mapped 66 QTLs,these detected in both F₂ and F_2:3 populations with high contribution rate include qPH-10, qFLW-4, qTGW-2,of which the positive alleles were also contributed by Zhongjiazao 17. In total, 66 QTLs for yield related traits were preliminarily examined in super rice variety Zhongjiazao 17, which will provide theoretical and technical supports to breed new super rice varieties with higher yield and better comprehensive characteristics by transfering excellent genes from Zhongjiazao 17 into other rice varieties.

Key words: QTL mapping, yield related trait, super rice

中图分类号:

圣忠华, 朱子亮, 马宁, 李威, 贺记外, 魏祥进, 邵高能, 王建龙, 胡培松, 唐绍清. 超级稻品种中嘉早17产量相关性状QTL定位研究[J]. 中国水稻科学, 2016, 30(1): 35-43.

Zhong-hua SHENG, Zi-liang ZHU, Ning MA, Wei LI, Ji-wai HE, Xiang-jin WEI, Gao-neng SHAO, Jian-long WANG, Pei-song HU, Shao-qing TANG. QTL Mapping of Yield Related Traits in Super Rice Variety Zhongjiazao 17[J]. Chinese Journal OF Rice Science, 2016, 30(1): 35-43.

图/表 5

图1 中嘉早17/D50 F2与F2:3群体农艺性状表型频率分布 YK17-中嘉早17。

Fig. 1. Phenotypic frequency distributions of agronomic traits in F2 and F2:3 populations of Zhongjiazao 17 × D50. YK17, Zhongjiazao 17.

表1 中嘉早17/D50 F2与F2:3群体及亲本的农艺性状表型

Table 1 Agronomic traits performances of F2 and F2:3 populations of Zhongjiazao 17 × D50.

性状与世代 Trait and generation	F₂ / F_2:3分离群体 Segregation populations						亲本 Parent
性状与世代 Trait and generation	变幅 Variation			$X ̅$ ±SD	峰值 Peak	偏值 Partial	D50	中嘉早17 Zhongjiazao 17
株高 PH/cm
F₂	67.13~123.00	92.52±0.66	0.18	0.15		90.50		77.71
F_2:3	71.76~130.70	101.92±0.71	0.37	-0.26		91.20		79.30
剑叶长 LFL/cm
F₂	20.14~53.89	34.04±0.34	0.83	0.30		31.40		30.80
F_2:3	19.10~44.54	31.76±0.32	-0.10	0.16		33.10		30.30
剑叶宽 WFL/cm
F₂	1.06~2.23	1.65±0.01	0.20	0.01		1.31		1.79
F_2:3	1.18~3.77	1.66±0.02	2.26	3.52		1.35		1.81
有效穗数 NTPP
F₂	5.50~36.00	14.12±0.35	1.98	1.05		13.60		15.10
F_2:3	4.38~12.00	7.62±0.10	-0.13	0.52		12.80		15.60
穗长 PL/cm
F₂	18.56~33.25	25.54±0.17	0.52	-0.17		26.86		20.74
F_2:3	17.21~26.44	22.00±0.11	0.10	-0.30		27.10		19.80
单株总粒数 NFGP
F₂	897.00~4295.00	1654.35±33.24	5.28	1.69		2188.43		1586.63
F_2:3	594.88~1747.88	1076.50±15.30	-0.01	0.52		1785.30		1521.36
结实率 SSR
F₂	0.09~0.88	0.57±0.01	1.24	-0.56		62.29		71.30
F_2:3	0.20~0.87	0.63±0.01	1.14	-0.64		65.30		78.10
千粒重 TGW/g
F₂	15.17~29.00	22.97±0.15	0.44	0.06		22.00		25.60
F_2:3	18.25~33.25	27.09±0.17	0.23	-0.17		22.50		26.20

表1 中嘉早17/D50 F2与F2:3群体及亲本的农艺性状表型

Table 1 Agronomic traits performances of F2 and F2:3 populations of Zhongjiazao 17 × D50.

性状与世代 Trait and generation	F₂ / F_2:3分离群体 Segregation populations						亲本 Parent
性状与世代 Trait and generation	变幅 Variation			$X ̅$ ±SD	峰值 Peak	偏值 Partial	D50	中嘉早17 Zhongjiazao 17
株高 PH/cm
F₂	67.13~123.00	92.52±0.66	0.18	0.15		90.50		77.71
F_2:3	71.76~130.70	101.92±0.71	0.37	-0.26		91.20		79.30
剑叶长 LFL/cm
F₂	20.14~53.89	34.04±0.34	0.83	0.30		31.40		30.80
F_2:3	19.10~44.54	31.76±0.32	-0.10	0.16		33.10		30.30
剑叶宽 WFL/cm
F₂	1.06~2.23	1.65±0.01	0.20	0.01		1.31		1.79
F_2:3	1.18~3.77	1.66±0.02	2.26	3.52		1.35		1.81
有效穗数 NTPP
F₂	5.50~36.00	14.12±0.35	1.98	1.05		13.60		15.10
F_2:3	4.38~12.00	7.62±0.10	-0.13	0.52		12.80		15.60
穗长 PL/cm
F₂	18.56~33.25	25.54±0.17	0.52	-0.17		26.86		20.74
F_2:3	17.21~26.44	22.00±0.11	0.10	-0.30		27.10		19.80
单株总粒数 NFGP
F₂	897.00~4295.00	1654.35±33.24	5.28	1.69		2188.43		1586.63
F_2:3	594.88~1747.88	1076.50±15.30	-0.01	0.52		1785.30		1521.36
结实率 SSR
F₂	0.09~0.88	0.57±0.01	1.24	-0.56		62.29		71.30
F_2:3	0.20~0.87	0.63±0.01	1.14	-0.64		65.30		78.10
千粒重 TGW/g
F₂	15.17~29.00	22.97±0.15	0.44	0.06		22.00		25.60
F_2:3	18.25~33.25	27.09±0.17	0.23	-0.17		22.50		26.20

表2 中嘉早17/D50 F2与F2:3群体不同性状间的相关系数

Table 2 Correlation coefficients among agronomic traits in F2 and F2:3 populations of Zhongjiazao 17 × D50.

性状Trait (F₂)	株高 PH	剑叶长 LFL	剑叶宽 WFL	有效穗数 NTPP	穗长 PL	单株总粒数 NFGP	结实率 SSR	千粒重 TGW	性状Trait (F_2:3)
株高PH		0.322^**	0.242^**	0.004	0.066	-0.009	-0.008	0.080	株高PH
剑叶长LFL	0.323^**		0.211^**	-0.009	0.049	0.003	-0.035	-0.038	剑叶长LFL
剑叶宽WFL	0.407^**	0.271^**		-0.066	0.070	0.023	-0.119^*	0.036	剑叶宽WFL
有效穗数NTPP	-0.099	-0.014	-0.107		-0.184^**	0.671^**	-0.002	0.111	有效穗数NTPP
穗长PL	0.268^**	0.153^**	0.087	-0.158^**		0.205^**	-0.151^**	-0.073	穗长PL
单株总粒数NFGP	-0.010	0.003	-0.152^**	0.312^**	0.123^*		-0.119^*	-0.013	单株总粒数NFGP
结实率SSR	0.014	0.026	-0.022	-0.213^**	0.050	-0.260^**		0.118^*	结实率SSR
千粒重TGW	0.084	-0.077	0.081	-0.136^*	-0.008	-0.055	-0.070		千粒重TGW

表3 中嘉早17/D50 F2与F2:3群体检测到的产量相关性状QTL分布情况

Table 3 QTL distributions of yield related traits detected in F2 and F2:3 populations of Zhongjiazao 17 × D50.

性状 Trait	QTL	染色体 Chromosome	标记区间 Mapping interval	LOD值 LOD value			加性效应 Additive effect			贡献率 Contribute rate/%
性状 Trait	QTL	染色体 Chromosome	标记区间 Mapping interval	F₂		F_2:3		F₂	F_2:3	F₂	F_2:3
株高PH	qPH-1	1	RM3148-RM3746	4.18	2.76	-4.96	-3.02		11.68		4.25
	qPH-3-1	3	RM7576-RM14898		3.86		-4.77				9.63
	qPH-3-2	3	RM1373-RM7389	12.22	6.97	5.62	4.33		14.62		7.87
	qPH-4-1	4	RM6659-RM16759		2.60		-2.72				3.51
	qPH-4-2	4	RM16759-RM16852		2.59		-2.54				3.07
	qPH-5	5	RM163-RM3170	2.75	3.25	-2.37	-1.97		2.73		1.76
	qPH-6	6	RM162-RM5463	3.44	6.23	-3.70	-5.65		7.39		15.34
	qPH-7	7	RM118-RM248	5.79	2.67	2.85	2.41		4.28		2.97
	qPH-10	10	RM25366-RM25664	3.86	4.42	4.36	4.91		9.46		11.37
	qPH-12	12	RM28607-RM1227		2.76		-3.28				4.99
剑叶长LFL	qFLL-1	1	RM3746-RM8071		4.59		-2.36				12.08
	qFLL-2	2	RM6-RM6307	4.77	6.63	-1.87	-2.26		6.63		11.01
	qFLL-3	3	RM3329-RM7389		3.56		0.29				0.18
	qFLL-4	4	RM17377-RM17626		3.59		1.50				4.93
	qFLL-5-1	5	RM163-RM3790	4.71		-1.12			1.65
	qFLL-5-2	5	RM3790-RM5907	3.24		1.99			7.09
	qFLL-7-1	7	RM11-RM234		5.99		1.95				8.31
	qFLL-7-2	7	RM118-RM248	3.25	2.61	1.76	1.06		6.27		2.26
	qFLL-10	10	RM1375-RM3451		2.52		-1.53				5.03
	qFLL-11	11	RM286-RM332		2.52		1.24				3.40
	qFLL-12-1	12	RM3747-RM247		2.66		0.86				1.78
	qFLL-12-2	12	RM247-RM519		3.10		0.65				1.04
剑叶宽WFL	qFLW-1-1	1	RM1329-RM1344	3.08		-0.09			10.31
	qFLW-1-2	1	RM1344-RM8071	4.30		-0.10			14.10
	qFLW-1-3	1	RM212-RM3520	3.69		-0.09			11.08
	qFLW-3	3	RM3199-RM3684	2.50		-0.07			5.90
	qFLW-4	4	RM17377-RM17626	8.10	4.76	0.10	0.12		14.37		9.04
	qFLW-10	10	RM25366-RM25664		5.85		0.20				20.45
	qFLW-11	11	RM202-RM26652	2.84		0.02			0.90
	qFLW-12	12	RM519-RM28546	2.65		0.02			0.48
有效穗数NTPP	qETP-1-1	1	RM1329-RM3746		3.64		0.56				5.44
	qETP-1-2	1	RM3746-RM1344		4.38		0.85				9.72
	qETP-1-3	1	RM8071-RM493		2.81		-0.10				0.16
	qETP-6	6	RM20522-RM5463	3.15		2.37			9.41
	qETP-7	7	RM234-RM118	3.05		2.55			8.15
	qETP-10	10	RM25366-RM25664	2.98		-1.72			5.48
穗长PL	qPL-1-1	1	RM3148-RM1329		4.89		-0.51				4.45
	qPL-1-2	1	RM212-RM3520		3.88		0.61				7.08
	qPL-2	2	RM6307-RM7337	6.51		-1.50			17.60
	qPL-3-1	3	RM7576-RM14898		2.97		-0.77				9.32
	qPL-3-2	3	RM6929-RM6914		3.07		-0.35				1.25
	qPL-3-3	3	RM3329-RM7389	2.54	2.77	0.21	0.83		0.34		7.48
	qPL-4	4	RM3708-RM17303	4.62		0.60			2.88
	qPL-6-1	6	RM20069-RM20155	2.50		-0.64			2.96
	qPL-6-2	6	RM20155-RM162	2.75		-0.83			5.13
	qPL-6-3	6	RM3765-RM5463		2.81		-0.62				6.73
	qPL-7	7	RM118-RM248	5.45		0.53			2.32
	qPL-10	10	RM25366-RM25664	3.09		0.83			5.66
单株总粒数NFGP	qNGP-3-1	3	RM5480-RM7197		3.28		-106.49				9.90
	qNGP-3-2	3	RM15012-RM3601		3.66		91.77				7.67
	qNGP-6	6	RM3183-RM20069		3.10		-96.60				8.69
	qNGP-7	7	RM234-RM118	2.71		238.25			7.97
	qNGP-12	12	RM1300-RM1227		2.53		-59.34				3.31
结实率 SSR	qSSR-1	1	RM8068-RM3746		3.98		0.04				7.01
	qSSR-3-1	3	RM6914-RM15490		2.92		-0.05				8.41
	qSSR-3-2	3	RM3199-RM3684	3.17		-0.05			7.92
	qSSR-4	4	RM3708-RM3839	3.01		0.02			1.65
	qSSR-5	5	RM6841-RM3170	4.97		-0.06			12.42
	qSSR-6	6	RM20522-RM5463	3.01		-0.06			9.37
	qSSR-8	8	RM22529-RM23174		2.94		-0.04				6.31
	qSSR-11	11	RM26062-RM26343		3.54		-0.06				13.25
千粒重 TGW	qTGW-2	2	RM12495-RM341	7.91	2.93	1.17	1.09		14.91		9.60
	qTGW-3	3	RM15012-RM15844	8.44	3.64	1.36	0.84		17.33		5.83
	qTGW-6	6	RM3805-RM20155	3.63	3.61	0.83	1.18		7.07		11.33
	qTGW-10	10	RM24952-RM2504	3.20		0.88			7.56
	qTGW-12	12	RM519-RM28546	2.64		-0.09			0.08

表3 中嘉早17/D50 F2与F2:3群体检测到的产量相关性状QTL分布情况

Table 3 QTL distributions of yield related traits detected in F2 and F2:3 populations of Zhongjiazao 17 × D50.

性状 Trait	QTL	染色体 Chromosome	标记区间 Mapping interval	LOD值 LOD value			加性效应 Additive effect			贡献率 Contribute rate/%
性状 Trait	QTL	染色体 Chromosome	标记区间 Mapping interval	F₂		F_2:3		F₂	F_2:3	F₂	F_2:3
株高PH	qPH-1	1	RM3148-RM3746	4.18	2.76	-4.96	-3.02		11.68		4.25
	qPH-3-1	3	RM7576-RM14898		3.86		-4.77				9.63
	qPH-3-2	3	RM1373-RM7389	12.22	6.97	5.62	4.33		14.62		7.87
	qPH-4-1	4	RM6659-RM16759		2.60		-2.72				3.51
	qPH-4-2	4	RM16759-RM16852		2.59		-2.54				3.07
	qPH-5	5	RM163-RM3170	2.75	3.25	-2.37	-1.97		2.73		1.76
	qPH-6	6	RM162-RM5463	3.44	6.23	-3.70	-5.65		7.39		15.34
	qPH-7	7	RM118-RM248	5.79	2.67	2.85	2.41		4.28		2.97
	qPH-10	10	RM25366-RM25664	3.86	4.42	4.36	4.91		9.46		11.37
	qPH-12	12	RM28607-RM1227		2.76		-3.28				4.99
剑叶长LFL	qFLL-1	1	RM3746-RM8071		4.59		-2.36				12.08
	qFLL-2	2	RM6-RM6307	4.77	6.63	-1.87	-2.26		6.63		11.01
	qFLL-3	3	RM3329-RM7389		3.56		0.29				0.18
	qFLL-4	4	RM17377-RM17626		3.59		1.50				4.93
	qFLL-5-1	5	RM163-RM3790	4.71		-1.12			1.65
	qFLL-5-2	5	RM3790-RM5907	3.24		1.99			7.09
	qFLL-7-1	7	RM11-RM234		5.99		1.95				8.31
	qFLL-7-2	7	RM118-RM248	3.25	2.61	1.76	1.06		6.27		2.26
	qFLL-10	10	RM1375-RM3451		2.52		-1.53				5.03
	qFLL-11	11	RM286-RM332		2.52		1.24				3.40
	qFLL-12-1	12	RM3747-RM247		2.66		0.86				1.78
	qFLL-12-2	12	RM247-RM519		3.10		0.65				1.04
剑叶宽WFL	qFLW-1-1	1	RM1329-RM1344	3.08		-0.09			10.31
	qFLW-1-2	1	RM1344-RM8071	4.30		-0.10			14.10
	qFLW-1-3	1	RM212-RM3520	3.69		-0.09			11.08
	qFLW-3	3	RM3199-RM3684	2.50		-0.07			5.90
	qFLW-4	4	RM17377-RM17626	8.10	4.76	0.10	0.12		14.37		9.04
	qFLW-10	10	RM25366-RM25664		5.85		0.20				20.45
	qFLW-11	11	RM202-RM26652	2.84		0.02			0.90
	qFLW-12	12	RM519-RM28546	2.65		0.02			0.48
有效穗数NTPP	qETP-1-1	1	RM1329-RM3746		3.64		0.56				5.44
	qETP-1-2	1	RM3746-RM1344		4.38		0.85				9.72
	qETP-1-3	1	RM8071-RM493		2.81		-0.10				0.16
	qETP-6	6	RM20522-RM5463	3.15		2.37			9.41
	qETP-7	7	RM234-RM118	3.05		2.55			8.15
	qETP-10	10	RM25366-RM25664	2.98		-1.72			5.48
穗长PL	qPL-1-1	1	RM3148-RM1329		4.89		-0.51				4.45
	qPL-1-2	1	RM212-RM3520		3.88		0.61				7.08
	qPL-2	2	RM6307-RM7337	6.51		-1.50			17.60
	qPL-3-1	3	RM7576-RM14898		2.97		-0.77				9.32
	qPL-3-2	3	RM6929-RM6914		3.07		-0.35				1.25
	qPL-3-3	3	RM3329-RM7389	2.54	2.77	0.21	0.83		0.34		7.48
	qPL-4	4	RM3708-RM17303	4.62		0.60			2.88
	qPL-6-1	6	RM20069-RM20155	2.50		-0.64			2.96
	qPL-6-2	6	RM20155-RM162	2.75		-0.83			5.13
	qPL-6-3	6	RM3765-RM5463		2.81		-0.62				6.73
	qPL-7	7	RM118-RM248	5.45		0.53			2.32
	qPL-10	10	RM25366-RM25664	3.09		0.83			5.66
单株总粒数NFGP	qNGP-3-1	3	RM5480-RM7197		3.28		-106.49				9.90
	qNGP-3-2	3	RM15012-RM3601		3.66		91.77				7.67
	qNGP-6	6	RM3183-RM20069		3.10		-96.60				8.69
	qNGP-7	7	RM234-RM118	2.71		238.25			7.97
	qNGP-12	12	RM1300-RM1227		2.53		-59.34				3.31
结实率 SSR	qSSR-1	1	RM8068-RM3746		3.98		0.04				7.01
	qSSR-3-1	3	RM6914-RM15490		2.92		-0.05				8.41
	qSSR-3-2	3	RM3199-RM3684	3.17		-0.05			7.92
	qSSR-4	4	RM3708-RM3839	3.01		0.02			1.65
	qSSR-5	5	RM6841-RM3170	4.97		-0.06			12.42
	qSSR-6	6	RM20522-RM5463	3.01		-0.06			9.37
	qSSR-8	8	RM22529-RM23174		2.94		-0.04				6.31
	qSSR-11	11	RM26062-RM26343		3.54		-0.06				13.25
千粒重 TGW	qTGW-2	2	RM12495-RM341	7.91	2.93	1.17	1.09		14.91		9.60
	qTGW-3	3	RM15012-RM15844	8.44	3.64	1.36	0.84		17.33		5.83
	qTGW-6	6	RM3805-RM20155	3.63	3.61	0.83	1.18		7.07		11.33
	qTGW-10	10	RM24952-RM2504	3.20		0.88			7.56
	qTGW-12	12	RM519-RM28546	2.64		-0.09			0.08

图2 中嘉早17/D50 F2与F2:3群体均检测到的产量相关性状QTL及其所包含的已被精细定位和克隆的产量相关基因在染色体上的分布情况

Fig. 2. QTLs distribution of yield related traits on chromosomes detected both in F2 and F2:3 populations of Zhongjiazao 17× D50, and some fine mapped or cloned yield related genes in the mapped QTL intervals.

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